Methods of miRNA analysis
MicroRNAs are a class of short RNA molecules that regulate gene expression at the post-transcriptional level. These molecules are involved in the various aspects of cell biology such as differentiation, proliferation, motility and formation of intercellular contacts, aging and cell death. Altered composition and functional activity of these molecules are observed in tumor cells. Therefore, analysis of the microRNA expression "profile" is a potential method for cancer diagnostics or evaluation of the clinically significant tumors characteristics. In addition, modification of the cellular microRNAs content is being investigated as a promising method of antitumor therapy.
PROJECTS:
- Involvement of cellular miRNAs in response of breast cancer cell to antitumor therapy
- Development of new method of cellular and extracellular (circulating) miRNAs isolation and analysis
- Development of new method of vesicular miRNAs isolation and analysis
STANDARD METHODS:
- Isolation of small RNAs using polymer-containing sorbents and magnetic particles
- Quantitative analysis by RT-PCR
NEW TECHNOLOGIES:
- Proteolysis as a method of miRNA isolation
- Quantitative analysis of microRNA by the two-tailed RT-qPCR
- Algorithms for identification and evaluation of the reciprocally dysregulated miRNA as a method for miRNA expression data processing and interpretation.
FUNDING: Research is carried out within the framework of Ministry of Health Assignment No. 056-00108-21-00 dated December 23, 2020 as a research project: "Development and clinical testing of methods for diagnosing and predicting the effect of cancer therapy based on microRNA analysis in biopsy material"
INVOLVEMENT OF CELLULAR MIRNAS IN RESPONSE OF BREAST CANCER CELL TO ANTITUMOR THERAPY
Investigation of the miRNAs role in the breast cancer cells response to the cytostatic agents (taxanes) and estrogen antagonists (tamoxifen), the development of (1) methods for prediction of the therapy effect and (2) methods for increasing sensitivity to therapy by modifying the cellular miRNAs composition.
The project is carried out in cooperation with the Department of Breast Tumors
Investigator: Sidina Elena
DEVELOPMENT OF NEW METHOD OF CELLULAR AND EXTRACELLULAR (CIRCULATING) MIRNAS ISOLATION AND ANALYSIS
Development of methods for isolation and analysis of cellular and extracellular circulating microRNAs from the tissue and liquid biopsies. Creation of methods for oncological diseases (cervical dysplasia, endometrial ectopia, germ cell tumors) diagnostics and monitoring based on the developed technologies.
The project is carried out in cooperation with the departments of gynecological oncology and chemotherapy and innovative technologies.
Investigator: Knyazeva Margo.
DEVELOPMENT OF NEW METHOD OF VESICULAR MIRNAS ISOLATION AND ANALYSIS
Development of methods for the isolation and analysis of vesicular miRNAs. Isolation of the tissue-specific populations of nanovesicles and assessment of their miRNA content. Development of methods for RT-PCR data normalization and analysis. Creation of methods for the diagnosis of follicular thyroid cancer and prostate cancer based on the described technologies.
The project is carried out in cooperation with the Department of Oncourology
Investigator: Zabegina Lidia
HEAD OF THE LAB:
RESEACH STAFF:
“Onco-endocrinology” team
Berstein L.M., MD., prof.
Tsyrlina E.V., MD., PhD.
Vasiliev D.A., MD., PhD.
Kovalenko I.M., MD., PhD
“Nanovesicles-related technology” team
Nazarova I.V.
Nikifiriva N.S.
“Methods of miRNA analysis” team:
Sidina E.I.
Knyazeva M.S.
Zabegina L.M.
Malysheva S.A.